1.1 Getting many diverse conformations routinely into well-diffracting crystals; and knowing how to interpret them biologically.
On 15/07/2019 20:44, Holton, James M wrote: > Hello folks, > > I have the distinct honor of chairing the next Gordon Research > Conference on Diffraction Methods in Structural Biology (July 26-31 > 2020). This meeting will focus on the biggest challenges currently > faced by structural biologists, and I mean actual real-world > challenges. As much as possible, these challenges will take the form of > friendly competitions with defined parameters, data, a scoring system, > and "winners", to be established along with other unpublished results > only at the meeting, as is tradition at GRCs. > > But what are the principle challenges in biological structure > determination today? I of course have my own ideas, but I feel like I'm > forgetting something. Obvious choices are: > 1) getting crystals to diffract better > 2) building models into low-resolution maps (after failing at #1) > 3) telling if a ligand is really there or not > 4) the phase problem (dealing with weak signal, twinning and > pseudotranslation) > 5) what does "resolution" really mean? > 6) why are macromolecular R factors so much higher than small-molecule ones? > 7) what is the best way to process serial crystallography data? > 8) how should one deal with non-isomorphism in multi-crystal methods? > 9) what is the "structure" of something that won't sit still? > > What am I missing? Is industry facing different problems than > academics? Are there specific challenges facing electron-based > techniques? If so, could the combined strength of all the world's > methods developers solve them? I'm interested in hearing the voice of > this community. On or off-list is fine. > > -James Holton > MAD Scientist > > > ######################################################################## > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
