I am afraid that R factor is not encoutaging! How molecules in the asymmeric unit? Eleanor
On Tue, 11 Jun 2019 at 01:03, Weixi Gu <weixi...@uq.net.au> wrote: > Hi Eleanor, > Thank you for your answers. I just want to add more information. > The resolution is ~ 2A. The space group is P1. After phenix refine, Rwork > decreases from 0.525 to 0.5175, but Rfree increases from 0.5239 to 0.5381. > Is the high LLG, low TFZ because of the wrong or bad search model? Thank > you! > Cheers, > Weixi > > ------------------------------ > *From:* Eleanor Dodson <eleanor.dod...@york.ac.uk> > *Sent:* Monday, 10 June 2019 9:55 PM > *To:* Weixi Gu > *Cc:* CCP4BB@JISCMAIL.AC.UK > *Subject:* Re: [ccp4bb] High top LLG, but low top TFZ > > Hardly enough information! > Resolution? > > Wrong spacegroup - eg P21212 when it should be P212121 or something? > > Wrong estimate of model difference? That can afffect LLG quite a lot? > > But there are many reasons.. > Does the Rfactor fall when you refine? A change of R=55% to R=47% usually > indicates the solution is somewhat correct and can be improved.. > > > On Mon, 10 Jun 2019 at 12:37, Weixi Gu <weixi...@uq.net.au> wrote: > > Dear all, > I have been using Phaser for molecular replacement. One of the search > models I tired generated 5 MR solutions. The best solution has a over 300 > LLG value, but a low TFZ value (5). I checked the map in coot. The density > and model didn't match well. I am wondering if it is normal? What's the > problem? > Thank you! > Cheers, > Weixi > > ------------------------------ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > > ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
