In all seriousness, it looks like it may be some form of hexose sugar? Tristan Croll Research Fellow Cambridge Institute for Medical Research University of Cambridge CB2 0XY
> On 2 Nov 2018, at 21:14, Deborah Harrus <deborah.har...@oulu.fi> wrote: > > Dear all, > > I came across an unidentified rabbit-head-shaped blob and would need help for > its identification. There are 2 molecules of protein per asymmetric unit but > there are some differences between the two chains. The blob is located in > between the two chains, and is surrounded by residues Asp, Pro, and Val. > > The protein, a glycosyltransferase, was expressed in E. coli BL21(DE3) and > purified on Ni-NTA followed by gel filtration. The purification buffer > included Sodium phosphate and NaCl. The crystallization condition had > Bis-Tris, ammonium acetate and PEG 20000, and glycerol was used as > cryoprotectant. From the size, bis-tris was the most probable, but I have > tried to fit it and it is not convincing. > > The structure is 1.6 angstrom resolution and this is the only thing left to > be done, it's driving me crazy! > > Pictures attached show the face, bottom and top of the head. 2Fo-Fc is at 1.1 > sigma, Fo-Fc at 3 sigmas. > > Many thanks in advance for your suggestions! > > Regards, > Deborah. > > > ================================================= > Deborah Harrus, PhD. > > University of Oulu / Faculty of Biochemistry and Molecular Medicine > Aapistie 7 A, 90220 Oulu > Finland > > office: F123B > email: deborah.har...@oulu.fi > phone: +358 50 3502387 / +358 44 2386351 > http://www.oulu.fi/fbmm/node/20603 > ================================================= > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > > <rabbit3.jpg> > <rabbit4.jpg> > <rabbit5.jpg> ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
