Fig. 2.13 in Gale Rhodes’ “Crystallography Made Crystal Clear” touches on rabbit heads and diffraction, and is one of my favorite cartoons (sadly it doesn’t directly address your problem).
> On 2 Nov 2018, at 5:14 PM, Deborah Harrus <deborah.har...@oulu.fi> wrote: > > Dear all, > > I came across an unidentified rabbit-head-shaped blob and would need help for > its identification. There are 2 molecules of protein per asymmetric unit but > there are some differences between the two chains. The blob is located in > between the two chains, and is surrounded by residues Asp, Pro, and Val. > > The protein, a glycosyltransferase, was expressed in E. coli BL21(DE3) and > purified on Ni-NTA followed by gel filtration. The purification buffer > included Sodium phosphate and NaCl. The crystallization condition had > Bis-Tris, ammonium acetate and PEG 20000, and glycerol was used as > cryoprotectant. From the size, bis-tris was the most probable, but I have > tried to fit it and it is not convincing. > > The structure is 1.6 angstrom resolution and this is the only thing left to > be done, it's driving me crazy! > > Pictures attached show the face, bottom and top of the head. 2Fo-Fc is at 1.1 > sigma, Fo-Fc at 3 sigmas. > > Many thanks in advance for your suggestions! > > Regards, > Deborah. > > > ================================================= > Deborah Harrus, PhD. > > University of Oulu / Faculty of Biochemistry and Molecular Medicine > Aapistie 7 A, 90220 Oulu > Finland > > office: F123B > email: deborah.har...@oulu.fi > phone: +358 50 3502387 / +358 44 2386351 > http://www.oulu.fi/fbmm/node/20603 > ================================================= > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > <rabbit3.jpg><rabbit4.jpg><rabbit5.jpg> --------------------------------------------------------------------------------------- Patrick J. Loll, Ph. D. Professor of Biochemistry & Molecular Biology Drexel University College of Medicine Room 10-102 New College Building 245 N. 15th St., Mailstop 497 Philadelphia, PA 19102-1192 USA (215) 762-7706 pjl...@gmail.com pj...@drexel.edu ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
