Dear Liang, The first thing I would do is to look through your complete scan. ADXV has an option to display a movie of all images. It might be that some regions of your scan are very weak or otherwise bad. Merging these regions with good regions will produce high Rmerges. I would also check for overloads and/or ice rings. These may wreak havoc on your scaling which, again, may produce very high Rmerges. I would also try some other processing program (xds, mosflm, dials….), maybe one of these does not suffer from the scaling problem. You should also process maybe just the first 30 or 40 frames to see what kind of Rmerges come out then.
Good luck! Herman Von: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] Im Auftrag von Zhang Foggy Gesendet: Freitag, 28. September 2018 10:10 An: CCP4BB@JISCMAIL.AC.UK Betreff: [EXTERNAL] [ccp4bb] R-merge is too high !! Dear All, Sorry for the off-topic. I recently collected a set of data. The diffraction spots are extremely sharp. However, When I used HKL3000 to scale it, I get a final resolution at 3.1A with overall R-merge ~0.54 (R-merge in the highest 3.2A-3.1A shell: 1.59). Then I solve the structure with final R value 0.19 and R free value 0.24 although I know this Rmerge value is totally unacceptable, and the density looks perfect. I also tried to collect other four set of data with different crystals. unfortunately, all of them have same problem. I ask one of my friend who is an expert in HKL3000, but he had no idea about it. Does anyone has suggestions? Here is the scale information for your review: Space group: P43 (I also tried P1, the Rmerge value is still similar) Shell Lower Upper Average Average Norm. Linear Square limit Angstrom I error stat. Chi**2 R-fac R-fac Rmeas Rpim CC1/2 CC* 50.00 6.67 11.6 0.9 0.3 1.165 0.191 0.284 0.198 0.052 0.975 0.994 6.67 5.30 4.5 0.5 0.3 0.952 0.317 0.313 0.329 0.086 0.971 0.993 5.30 4.63 7.3 0.7 0.5 0.961 0.293 0.297 0.304 0.081 0.975 0.994 4.63 4.21 7.0 0.8 0.6 0.986 0.369 0.358 0.382 0.101 0.969 0.992 4.21 3.91 5.6 0.8 0.6 1.040 0.522 0.491 0.541 0.142 0.955 0.988 3.91 3.68 4.6 0.9 0.7 1.064 0.718 0.669 0.746 0.203 0.929 0.981 3.68 3.49 3.5 0.9 0.8 1.092 1.059 0.986 1.101 0.299 0.882 0.968 3.49 3.34 2.6 0.9 0.8 1.092 1.382 1.298 1.438 0.395 0.829 0.952 3.34 3.21 2.1 0.9 0.8 1.084 1.543 1.489 1.614 0.468 0.772 0.933 3.21 3.10 1.6 0.9 0.8 1.070 1.591 1.669 1.680 0.529 0.645 0.885 All reflections 5.0 0.8 0.6 1.048 0.538 0.487 0.559 0.153 Thank you. Liang ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1<https://urldefense.proofpoint.com/v2/url?u=https-3A__www.jiscmail.ac.uk_cgi-2Dbin_webadmin-3FSUBED1-3DCCP4BB-26A-3D1&d=DwMGaQ&c=Dbf9zoswcQ-CRvvI7VX5j3HvibIuT3ZiarcKl5qtMPo&r=HK-CY_tL8CLLA93vdywyu3qI70R4H8oHzZyRHMQu1AQ&m=WqYzDHm60h9i9RU9AjHUHyL05-u0rhUZd-yPqex3JHk&s=w28a0GfmgoXbK8ZZBaoDjrOvF7I_nSGA32mbj8J7Eac&e=> ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
