[cid:a3a4faea-08e2-46af-88de-3bde456d9124@fed.cclrc.ac.uk]
Sorry for spamming you all on this, but these images keep on getting stripped for Armin and myself. On 2 Aug 2018, at 16:51, Charles Ballard - UKRI STFC wrote: > Begin forwarded message (on behalf of Armin Wagner) > From: ahw78 <armin.wag...@diamond.ac.uk<mailto:armin.wag...@diamond.ac.uk>> > Date: 2 August 2018 at 09:17:48 BST > To: Eleanor Dodson > <eleanor.dod...@york.ac.uk<mailto:eleanor.dod...@york.ac.uk>> > Cc: ccp4bb <ccp4bb@jiscmail.ac.uk<mailto:ccp4bb@jiscmail.ac.uk>> > Subject: Re: [ccp4bb] Sulphate or phosphate? > Dear Eleanor, > > We can tune the wavelength over a large range (lambda = 1.2 – 6 A). This > allows collecting data above and below the absorption edges of Ca, K, Cl, S > and even P. Crystal, solvent and loop absorption are of course a problem for > the longest wavelengths, but so far we have done quite a fair amount of > studies around these absorption edges with strong signal above the edge and > obviously no signal below. Resolution in our setup is limited to around 3.2 A > at 5 A wavelength, but for anomalous difference fourier maps this is > sufficient. I have attached an example for a disulphide bridge which should > illustrate it better. > > Best regards, > > Armin > > > > [cid:image001.png@01D42A41.AE0B8B60] > > From: Eleanor Dodson > <eleanor.dod...@york.ac.uk<mailto:eleanor.dod...@york.ac.uk>> > Date: Wednesday, 1 August 2018 at 19:09 > To: Armin Wagner > <armin.wag...@diamond.ac.uk<mailto:armin.wag...@diamond.ac.uk>> > Cc: "ccp4bb@jiscmail.ac.uk<mailto:ccp4bb@jiscmail.ac.uk>" > <ccp4bb@jiscmail.ac.uk<mailto:ccp4bb@jiscmail.ac.uk>> > Subject: Re: [ccp4bb] Sulphate or phosphate? > > I am not sure if you gave your wave length but it is always worth doing an > anomalous map, and looking at relativr peak heights for your known S > positions and the putative sulphate or phosphate. > There are small differences in the expected f" at most wavelengths. > > Chemical arguments are doubtless better of Course! > Eleanor > > On 1 August 2018 at 16:54, > armin.wag...@diamond.ac.uk<mailto:armin.wag...@diamond.ac.uk> > <armin.wag...@diamond.ac.uk<mailto:armin.wag...@diamond.ac.uk>> wrote: > Dear David, > > The long-wavelength beamline I23 at Diamond > (http://www.diamond.ac.uk/Instruments/Mx/I23.html) can go all way down to the > phosphorous edge. While data quality will be obviously compromised by > absorption effects (we are working on this), for a reasonably well > diffracting crystal like yours we should be able to get anomalous difference > fourier maps from data collected above and below the sulphur edge (lambda ~5 > A) to answer your question. > > We’ve published some test crystal work around the sulphur edge a while ago, > you might want to have a look here: > https://doi.org/10.1016/j.nimb.2016.12.005 > > The beamline is currently in an advanced commissioning phase accepting a > limited number of projects. So, please get into touch if you are interested. > We are currently sorting out a few more hardware and software aspects before > we can run the beamline as a standard user facility, but we have made a lot > of progress over the past months. There will be more details here on the > bulletin board ahead of the next call for proposals in autumn. > > Best regards, > > Armin > > > Postdoc position for long-wavelength crystallography: > https://vacancies.diamond.ac.uk/vacancy/post-doctoral-research-associate-355285.html > > > > > On 31/07/2018, 14:38, "CCP4 bulletin board on behalf of David Schuller" > <CCP4BB@JISCMAIL.AC.UK<mailto:CCP4BB@JISCMAIL.AC.UK> on behalf of > schul...@cornell.edu<mailto:schul...@cornell.edu>> wrote: > > How can one distinguish between a sulphate or phosphate in an electron > density map? Both are present in the mother liquor, and resolution is in > the range of 1.75 - 2.25 A > > > -- > ======================================================================= > All Things Serve the Beam > ======================================================================= > David J. Schuller > modern man in a post-modern world > MacCHESS, Cornell University > > schul...@cornell.edu<mailto:schul...@cornell.edu> > > ######################################################################## > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > > > > -- > This e-mail and any attachments may contain confidential, copyright and or > privileged material, and are for the use of the intended addressee only. If > you are not the intended addressee or an authorised recipient of the > addressee please notify us of receipt by returning the e-mail and do not use, > copy, retain, distribute or disclose the information in or attached to the > e-mail. > Any opinions expressed within this e-mail are those of the individual and not > necessarily of Diamond Light Source Ltd. > Diamond Light Source Ltd. cannot guarantee that this e-mail or any > attachments are free from viruses and we cannot accept liability for any > damage which you may sustain as a result of software viruses which may be > transmitted in or with the message. > Diamond Light Source Limited (company no. 4375679). Registered in England and > Wales with its registered office at Diamond House, Harwell Science and > Innovation Campus, Didcot, Oxfordshire, OX11 0DE, United Kingdom > > > ######################################################################## > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > > [cid:image001.png@01D42A41.AE0B8B60] > > ######################################################################## > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
