Dear all,
Sorry, now with the attachment of an anomalous difference fourier above and
below the sulphur edge.
Best regards,
Armin
On 02/08/2018, 09:17, "CCP4 bulletin board on behalf of
armin.wag...@diamond.ac.uk" <CCP4BB@JISCMAIL.AC.UK on behalf of
armin.wag...@diamond.ac.uk> wrote:
Dear Eleanor,
We can tune the wavelength over a large range (lambda = 1.2 – 6 A). This
allows collecting data above and below the absorption edges of Ca, K, Cl, S and
even P. Crystal, solvent and loop absorption are of course a problem for the
longest wavelengths, but so far we have done quite a fair amount of studies
around these absorption edges with strong signal above the edge and obviously
no signal below. Resolution in our setup is limited to around 3.2 A at 5 A
wavelength, but for anomalous difference fourier maps this is sufficient. I
have attached an example for a disulphide bridge which should illustrate it
better.
Best regards,
Armin
[cid:image001.png@01D42A41.AE0B8B60]
From: Eleanor Dodson <eleanor.dod...@york.ac.uk>
Date: Wednesday, 1 August 2018 at 19:09
To: Armin Wagner <armin.wag...@diamond.ac.uk>
Cc: "ccp4bb@jiscmail.ac.uk" <ccp4bb@jiscmail.ac.uk>
Subject: Re: [ccp4bb] Sulphate or phosphate?
I am not sure if you gave your wave length but it is always worth doing an
anomalous map, and looking at relativr peak heights for your known S positions
and the putative sulphate or phosphate.
There are small differences in the expected f" at most wavelengths.
Chemical arguments are doubtless better of Course!
Eleanor
On 1 August 2018 at 16:54,
armin.wag...@diamond.ac.uk<mailto:armin.wag...@diamond.ac.uk>
<armin.wag...@diamond.ac.uk<mailto:armin.wag...@diamond.ac.uk>> wrote:
Dear David,
The long-wavelength beamline I23 at Diamond
(http://www.diamond.ac.uk/Instruments/Mx/I23.html) can go all way down to the
phosphorous edge. While data quality will be obviously compromised by
absorption effects (we are working on this), for a reasonably well diffracting
crystal like yours we should be able to get anomalous difference fourier maps
from data collected above and below the sulphur edge (lambda ~5 A) to answer
your question.
We’ve published some test crystal work around the sulphur edge a while ago,
you might want to have a look here:
https://doi.org/10.1016/j.nimb.2016.12.005
The beamline is currently in an advanced commissioning phase accepting a
limited number of projects. So, please get into touch if you are interested. We
are currently sorting out a few more hardware and software aspects before we
can run the beamline as a standard user facility, but we have made a lot of
progress over the past months. There will be more details here on the bulletin
board ahead of the next call for proposals in autumn.
Best regards,
Armin
Postdoc position for long-wavelength crystallography:
https://vacancies.diamond.ac.uk/vacancy/post-doctoral-research-associate-355285.html
On 31/07/2018, 14:38, "CCP4 bulletin board on behalf of David Schuller"
<CCP4BB@JISCMAIL.AC.UK<mailto:CCP4BB@JISCMAIL.AC.UK> on behalf of
schul...@cornell.edu<mailto:schul...@cornell.edu>> wrote:
How can one distinguish between a sulphate or phosphate in an electron
density map? Both are present in the mother liquor, and resolution is in
the range of 1.75 - 2.25 A
--
=======================================================================
All Things Serve the Beam
=======================================================================
David J. Schuller
modern man in a post-modern world
MacCHESS, Cornell University
schul...@cornell.edu<mailto:schul...@cornell.edu>
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