You might be able to distinguish sulfate from phosphate by examining hydrogen bonding partners. Phosphate can donate one or two hydrogen bonds at neutral pH values, whereas sulfate is usually only a hydrogen bond acceptor. (Having said that, we have published a structure where a sulfate clearly interacts with a glutamate, the later of which which may or may not be protonated at near neutral pH.)
_______________________________________ Roger S. Rowlett Gordon & Dorothy Kline Professor, Emeritus Department of Chemistry Colgate University 13 Oak Drive Hamilton, NY 13346 tel: (315)-723-7245 email: rrowl...@colgate.edu On Tue, Jul 31, 2018 at 9:38 AM, David Schuller <schul...@cornell.edu> wrote: > How can one distinguish between a sulphate or phosphate in an electron > density map? Both are present in the mother liquor, and resolution is in > the range of 1.75 - 2.25 A > > > -- > ======================================================================= > All Things Serve the Beam > ======================================================================= > David J. Schuller > modern man in a post-modern world > MacCHESS, Cornell University > schul...@cornell.edu > > ######################################################################## > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
