On a side note, I assume that you have searched with both P2 as well as P21 for your space group. If not, I will point out that there are 265 submissions in the rcsb for the P2 space group, compared to 20,698 for P21. That makes P2 fairly rare. Good Luck! - Todd
________________________________ Von: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] Im Auftrag von ??? Gesendet: Dienstag, 8. Mai 2018 16:41 An: CCP4BB@JISCMAIL.AC.UK<mailto:CCP4BB@JISCMAIL.AC.UK> Betreff: [EXTERNAL] [ccp4bb] tNCS problem Dear all, Recently, I collected a data set of a crystal of a big protein, which contains 1000 amino acids. I processed the data to P2. But the data set has tNCS problem. I want to do molecular replacement. Is there anyone know how to deal with this problem? Thanks, JiYG