Re: [ccp4bb] AW: [ccp4bb] tNCS problem

[苏纪勇]

Dear Herman, I tried phaser. It worked. But there are a lot of crashes in the structure. Meanwhile, R factors could not be lowered. I tried to use P1 to process the data, but I do not know how many monomers in the asymetric unit. Bests, JiYG 

On 05/08/2018 22:50, herman.schreu...@sanofi.com wrote:

Dear JiYG,

 

Unless the tNCS has caused processing problems, Phaser should automatically deal with tNCS and I would recommend to just give it a try. If it fails, you could try more sophisticated approaches.

 

Best,

Herman

 

Von: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] Im Auftrag von ???
Gesendet: Dienstag, 8. Mai 2018 16:41
An: CCP4BB@JISCMAIL.AC.UK
Betreff: [EXTERNAL] [ccp4bb] tNCS problem

 

Dear all, Recently, I collected a data set of a crystal of a big protein, which contains 1000 amino acids. I processed the data to P2. But the data set has tNCS problem. I want to do molecular replacement. Is there anyone know how to deal with this problem? Thanks, JiYG