Dear JiYG, Unless the tNCS has caused processing problems, Phaser should automatically deal with tNCS and I would recommend to just give it a try. If it fails, you could try more sophisticated approaches.
Best, Herman Von: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] Im Auftrag von ??? Gesendet: Dienstag, 8. Mai 2018 16:41 An: CCP4BB@JISCMAIL.AC.UK Betreff: [EXTERNAL] [ccp4bb] tNCS problem Dear all, Recently, I collected a data set of a crystal of a big protein, which contains 1000 amino acids. I processed the data to P2. But the data set has tNCS problem. I want to do molecular replacement. Is there anyone know how to deal with this problem? Thanks, JiYG
