Hi, I was going to suggest the same but since it has already been said, here’s a cheeky suggestion: you could try determining the crystal structure of the complex and get a direct sequence readout for the nuclei acid. Best, Debanu
> On Mar 5, 2018, at 9:34 PM, Philippe BENAS > <00000d88e888355a-dmarc-requ...@jiscmail.ac.uk> wrote: > > Hi Jobi, > > Phenol/CHCl3 extraction, iPrOH precipitation and then nucleic acid sequencing. > > Best regards, > Philippe > > Philippe BENAS, Ph.D. > > Laboratoire de Cristallographie et RMN Biologiques, UMR 8015 CNRS > Faculté de Pharmacie, Université Paris Descartes > Case 48 > Av, de l'Observatoire > F-75270 PARIS cedex 06 > +33.1.5373.1599 > E-mails: philippe.be...@parisdescartes.fr, philippe_be...@yahoo.fr > URLs: http://lcrbw.pharmacie.univ-paris5.fr/ , > http://lcrbw.pharmacie.univ-paris5.fr/spip.php?article18 > > > > De : Jobichen Chacko <jobich...@gmail.com> > À : CCP4BB@JISCMAIL.AC.UK > Envoyé le : Mardi 6 mars 2018 5h11 > Objet : [ccp4bb] Off-topic question > > Dear All, > We are trying to identify/sequence a DNA/RNA fragment (around 100bp) which > was co-purified along with our protein. > The expression was done in E.coli. > Any suggestions on how to do this. > Thank you. > Jobi > >
