Hi Jobi, Phenol/CHCl3 extraction, iPrOH precipitation and then nucleic acid sequencing.
Best regards,Philippe Philippe BENAS, Ph.D. Laboratoire de Cristallographie et RMN Biologiques, UMR 8015 CNRS Faculté de Pharmacie, Université Paris Descartes Case 48 Av, de l'Observatoire F-75270 PARIS cedex 06 +33.1.5373.1599 E-mails: philippe.be...@parisdescartes.fr, philippe_be...@yahoo.fr URLs: http://lcrbw.pharmacie.univ-paris5.fr/ , http://lcrbw.pharmacie.univ-paris5.fr/spip.php?article18 De : Jobichen Chacko <jobich...@gmail.com> À : CCP4BB@JISCMAIL.AC.UK Envoyé le : Mardi 6 mars 2018 5h11 Objet : [ccp4bb] Off-topic question Dear All, We are trying to identify/sequence a DNA/RNA fragment (around 100bp) which was co-purified along with our protein. The expression was done in E.coli. Any suggestions on how to do this. Thank you. Jobi
