Hi Vijay, Why there is no 2mFo-DFc (blue) feature on this mFo-DFc (green) map?!! Is the contour level for blue map set high?!! If there is no 2mFo-DFc density, should this be consider as noise?!!
Hope this helps, Best Wishes, Partha On Thu, Oct 26, 2017 at 10:23 AM, Vijaykumar Pillalamarri < vijaypkuma...@gmail.com> wrote: > Dear ccp4bb, > > I am solving the structure of a 1.6 Angstrom data of a metallo protein. > While everything else is straight forward, this density (see the picture) > seems unfamiliar to me. I don't see any thing fits in the density. This > density present just above Histidine-Cobalt. What ever I try to fit, the > molecule clahes with histidine ( I mean the distance between His and > molecule is <2 Angstrom). > > The crystallization condition is 0.1M Bistris, 19% PEG 3350 and 50% > glycerol. > > Any suggestions? > > Thanks, > Vijaykumar Pillalamarri > UGC-SRF > C/O: Dr. Anthony Addlagatta > Principal Scientist > CSIR-IICT, Tarnaka > Hyderabad, India-500007 > Mobile: +918886922975 <+91%2088869%2022975> >
