Dear Dr. Vaheh, I tried fitting waters around Co but the positive density is still present even after refinement.
Thanks, Vijaykumar On 26 October 2017 at 20:19, Oganesyan, Vaheh <oganesy...@medimmune.com> wrote: > Water molecules completing Co coordination? > > > > *Regards,* > > > > *Vaheh Oganesyan* > > *www.medimmune.com <http://www.medimmune.com>* > > > > *From:* CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] *On Behalf Of > *Vijaykumar > Pillalamarri > *Sent:* Thursday, October 26, 2017 10:24 AM > *To:* CCP4BB@JISCMAIL.AC.UK > *Subject:* [ccp4bb] Unknown electron density > > > > Dear ccp4bb, > > > > I am solving the structure of a 1.6 Angstrom data of a metallo protein. > While everything else is straight forward, this density (see the picture) > seems unfamiliar to me. I don't see any thing fits in the density. This > density present just above Histidine-Cobalt. What ever I try to fit, the > molecule clahes with histidine ( I mean the distance between His and > molecule is <2 Angstrom). > > > > The crystallization condition is 0.1M Bistris, 19% PEG 3350 and 50% > glycerol. > > > > Any suggestions? > > > > Thanks, > > Vijaykumar Pillalamarri > > UGC-SRF > > C/O: Dr. Anthony Addlagatta > > Principal Scientist > > CSIR-IICT, Tarnaka > > Hyderabad, India-500007 > > Mobile: +918886922975 > To the extent this electronic communication or any of its attachments > contain information that is not in the public domain, such information is > considered by MedImmune to be confidential and proprietary. This > communication is expected to be read and/or used only by the individual(s) > for whom it is intended. If you have received this electronic communication > in error, please reply to the sender advising of the error in transmission > and delete the original message and any accompanying documents from your > system immediately, without copying, reviewing or otherwise using them for > any purpose. Thank you for your cooperation. > -- Vijaykumar Pillalamarri UGC-SRF C/O: Dr. Anthony Addlagatta Principal Scientist CSIR-IICT, Tarnaka Hyderabad, India-500007 Mobile: +918886922975
