Hena There was a very interesting paper by Peter Sun and coworker from 2002. They pointed out that there is a very strong bias towards crystallizing protein-protein complexes with PEG rather than salt as the main precipitant.
Patrick _______________________________ Radaev and Sun. Crystallization of protein-protein complexes. J. Appl. Cryst. (2002). 35, 674-676 On 20 May 2017 at 22:14, Hena Dutta <hdutt...@gmail.com> wrote: > > Dear Members, > I am trying to crystallize a protein-protein complex. Both are soluble proteins and form complex as observed in FPLC either co-purifying or separately purifying and then mixing with equi-molar ratio. > Looking for suggested screens to try for crystallization. > > So far tried with Qiagen - ProComplex Suite, Classics Suite and Classics II Suite > No success yet. > > Is there any guidance which screen to try? > > Looking for your help... > Regards, > Hena. -- patr...@douglas.co.uk Douglas Instruments Ltd. Douglas House, East Garston, Hungerford, Berkshire, RG17 7HD, UK Directors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk Tel: 44 (0) 148-864-9090 US toll-free 1-877-225-2034 Regd. England 2177994, VAT Reg. GB 480 7371 36
