To add to Pavel’s comments, it is also possible to do the automated model building in Phenix:
http://www.phenix-online.org/documentation/reference/map_to_model.html As Pavel said: there is Phenix mailing list for Phenix-specific questions > On May 17, 2017, at 10:24 AM, Paul Emsley <pems...@mrc-lmb.cam.ac.uk> wrote: > > Hi, all. > > I have collected cryoEM data and want to use Coot and CCP4 program to build > model and to refine it. > > 1. What is the steps to do this? > 2. How do I convert cryoEM map file to MTZ file? > 3. Can I also use Phenix for this purpose? > > Thanks to all for your help in advance. -- Paul Adams Division Director, Molecular Biophysics & Integrated Bioimaging, Lawrence Berkeley Lab Division Deputy for Biosciences, Advanced Light Source, Lawrence Berkeley Lab Adjunct Professor, Department of Bioengineering, U.C. Berkeley Vice President for Technology, the Joint BioEnergy Institute Laboratory Research Manager, ENIGMA Science Focus Area Building 33, Room 347 Building 80, Room 247 Building 978, Room 4126 Tel: 1-510-486-4225, Fax: 1-510-486-5909 http://cci.lbl.gov/paul Lawrence Berkeley Laboratory 1 Cyclotron Road BLDG 33R0345 Berkeley, CA 94720, USA. Executive Assistant: Louise Benvenue [ lbenve...@lbl.gov ][ 1-510-495-2506 ] --