If your signal goes all the way to the half Nyquist frequency, oversample your map to have the pixel size equal to 1/3rd of the highest resolution. Your map will look much better in coot. Real space fitting will work much better as well.
Best, Petr > On May 17, 2017, at 11:24 AM, MyeongSeon Lee > <00000e01bd27cd0f-dmarc-requ...@jiscmail.ac.uk> wrote: > > Hi, all. > > I have collected cryoEM data and want to use Coot and CCP4 program to build > model and to refine it. > > 1. What is the steps to do this? > 2. How do I convert cryoEM map file to MTZ file? > 3. Can I also use Phenix for this purpose? > > Thanks to all for your help in advance.
