Dear Alex, Did you try freezing with the mother liquor as is? If you freeze fast enough, you may not need additional cryoprotectant with 25% PEG3350. You should first freeze some reservoir solution and look if it stays clear, before attempting to destroy a crystal.
Best, Herman Von: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] Im Auftrag von Alex Lee Gesendet: Mittwoch, 28. September 2016 00:43 An: CCP4BB@JISCMAIL.AC.UK Betreff: [ccp4bb] sodium thiocyanate as cryoprotectant Hi CCP4bb members, I have a condition for my protein crystals at "0.2M sodium thiocyanate, 25% PEG3350, pH6.9". I'd like to try cryoprotectant with just higher concentration of sodium thiocyanate, but I have no google hits of the range of concentration I should use. I wonder anyone in this forum tried to use sodium thiocyanate as cryoprotectant? What's the concentration range for this if used as cryoprotectant? Thanks.
