Hi Alex, You may also think of gradually increasing PEG3350 concentration to around 40% which has worked pretty well in some of my cases.
Greetings, Abhi Abhimanyu Kumar Singh, Ph.D. School of Biosciences University of Kent Canterbury, UK. On 27 Sep 2016 23:43, "Alex Lee" <alexlee198...@gmail.com> wrote: > Hi CCP4bb members, > > I have a condition for my protein crystals at "0.2M sodium thiocyanate, > 25% PEG3350, pH6.9". > > I'd like to try cryoprotectant with just higher concentration of sodium > thiocyanate, but I have no google hits of the range of concentration I > should use. > > I wonder anyone in this forum tried to use sodium thiocyanate as > cryoprotectant? What's the concentration range for this if used as > cryoprotectant? > > Thanks. >
