Additive screening
At 2015-04-11 18:00:21, "高艺娜" <gaoy...@cau.edu.cn> wrote: >Dear all >I have tried a variety of methods on regular optimization of the crystal ,but >all have failed,the 17 kDa protein crystal still have many nucleation and poor >diffraction also poor resolution(the best 3.5-4.0Å) >The crystallization conditions are: >0.1 M Tris pH-6.5, >0.2 M Sodium acetate >1.8 M Ammonium sulphate > >Could some one suggest me how to trouble shoot this problem? > >Best wishes,