Additive screening








At 2015-04-11 18:00:21, "高艺娜" <gaoy...@cau.edu.cn> wrote:
>Dear all
>I have tried a variety of methods on regular optimization of the crystal ,but  
>all have failed,the 17 kDa protein crystal still have many nucleation and poor 
>diffraction also poor resolution(the best 3.5-4.0Å)
>The crystallization conditions are:
>0.1 M Tris pH-6.5, 
>0.2 M Sodium acetate
>1.8 M Ammonium sulphate
>
>Could some one suggest me how to trouble shoot this problem?
>
>Best wishes,

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