Dear , If you want to get useful advice you have to give a bit more information. What did you try? Also, please do not attach large images to your message.
Thanks and good luck, bert ________________________________________ From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] on behalf of 高艺娜 [gaoy...@cau.edu.cn] Sent: Saturday, April 11, 2015 11:00 AM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] Problem in optimization Dear all I have tried a variety of methods on regular optimization of the crystal ,but all have failed,the 17 kDa protein crystal still have many nucleation and poor diffraction also poor resolution(the best 3.5－4.0Å） The crystallization conditions are: 0.1 M Tris pH-6.5, 0.2 M Sodium acetate 1.8 M Ammonium sulphate Could some one suggest me how to trouble shoot this problem? Best wishes,
