Dear Anita, As has been stated in another post I think, it is not a good idea to use a guard column in front of a Superdex column or any gel filtration column. It will dilute the sample and decrease the resolution and depending on the nature of the aggregate and guard column it might just clog. If you have aggregates, or suspect you have, installing an in-line filter will not help you in any other way than protecting the column. You need to remove the aggregates by ultracentrifugation beforehand and then make sure your sample volume is within the recommended load volume for the column (read the documentation that comes with column or get it from the GE Healthcare Lifesciences website) in order to get the resolution you paid GE for.
If you do not have any inline filters and you have a bunch of users with varied experience it might be a very good idea to install them and make sure that people run proper methods where the column has been selected to get the right max pressure settings. Compression of the matrix will also kill you column pretty nicely. Good Luck and Happy Hunting!!!! KM ----- Original Message ----- > From: "Anita P" <crystals...@gmail.com> > To: CCP4BB@JISCMAIL.AC.UK > Sent: Tuesday, September 16, 2014 1:29:44 AM > Subject: [ccp4bb] Guard columns from FPLC > > Hi All, > Sorry for this off topic. > > I have heard that there are these little columns called guard columns which > can be attached to AKTA purifiers. These columns prevent the incoming huge > aggregates to be deposited and blocking of the gel filtration columns. > > Can any one advice me regarding where to purchase these columns. I could > not find them in GE website. We have Superdex 16/60 on AKTA purifier. > > Thanks in advance. > Have a good day > > Anita > -- -------------------------------------- Karl-Magnus Larsson, Ph.D. Mail: Department of Structural Biology Fairchild Bldg D100 299 Campus Drive West Stanford University School of Medicine Stanford, CA 94305-5126 U.S.A ----- Visit: Beckman Center, Room B137 Stanford University School of Medicine 279 Campus Drive West Stanford, CA 94305 ------- Phone: +1-650-391-7408 (Cell)
