Hi Almudena,
you can also try mosflm as said Harry.
And you can try different setting in the XDS.INP, you can try to reduce the
STRONG_PIXEL (because you said spots are weak) number or/and
MINIMUM_NUMBER_OF_PIXELS_IN_A_SPOT (if the spot are small).
Nicolas
________________________________________
De : CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] de la part de Almudena Ponce
Salvatierra [maps.fa...@gmail.com]
Envoyé : lundi 19 mai 2014 18:18
À : CCP4BB@JISCMAIL.AC.UK
Objet : [ccp4bb] data processing with XDS
Dear all,
I am looking for some suggestions here. I have lately collected some datasets
but the spots are very very weak... it is very difficult to process them. At
times it looks like XDS is stalled or at times it just says that it can not
interpret the lattice parameters... also while running integrate I have such a
message after each range of images:
!!! WARNING !!! REFINEMENT DID NOT CONVERGE
LAST CORRECTION SHIFT WAS 9.1E-03 (should be < 1.0E-03)
I guess this is not good at all. And I wonder what to do? Is there any info
that I can get out from these data at all? or rather not?
I wonder if the problem is to find the spots, I have tried with HKL2000 and it
can't even find enough of them for indexing.
Any ideas are welcome.
Best wishes,
Almudena.
--
Almudena Ponce-Salvatierra
Macromolecular crystallography and Nucleic acid chemistry
Max Planck Institute for Biophysical Chemistry
Am Fassberg 11 37077 Göttingen
Germany
