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Dear Prerana,

is there a reason you do not try vapour diffusion instead of batch
crystallisation? You can have better control about the rate of change
which might affect the behaviour of the sample.

Regards,
Tim

On 02/24/2014 05:23 PM, Prerana G. wrote:
> Hi Vicky,
> 
> I tried to change the ratio of paraffin:silicon oil to 1:2, but
> still the protein precipitated. So I tried to do it other way
> round, I kept paraffin:silicon oil ratio as 2:1 and so far it has
> not precipitated. Apart from that, I separately used the two
> oils.In silicon oil there was immediate precipitation, but none in
> parafin oil.
> 
> 
> Prerana
> 

- -- 
- --
Dr Tim Gruene
Institut fuer anorganische Chemie
Tammannstr. 4
D-37077 Goettingen

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