-----BEGIN PGP SIGNED MESSAGE----- Hash: SHA1 Dear Prerana,
is there a reason you do not try vapour diffusion instead of batch crystallisation? You can have better control about the rate of change which might affect the behaviour of the sample. Regards, Tim On 02/24/2014 05:23 PM, Prerana G. wrote: > Hi Vicky, > > I tried to change the ratio of paraffin:silicon oil to 1:2, but > still the protein precipitated. So I tried to do it other way > round, I kept paraffin:silicon oil ratio as 2:1 and so far it has > not precipitated. Apart from that, I separately used the two > oils.In silicon oil there was immediate precipitation, but none in > parafin oil. > > > Prerana > - -- - -- Dr Tim Gruene Institut fuer anorganische Chemie Tammannstr. 4 D-37077 Goettingen GPG Key ID = A46BEE1A -----BEGIN PGP SIGNATURE----- Version: GnuPG v1.4.12 (GNU/Linux) Comment: Using GnuPG with Icedove - http://www.enigmail.net/ iD8DBQFTC3NoUxlJ7aRr7hoRAoV1AJ9zCduZyveucwdGnxXKc+ApXS48TwCfRs5Q Aov3mv3qrfP8LGFOc68uQNE= =0/pf -----END PGP SIGNATURE-----