Hi, You could change the ratio of paraffin and silicon oil (1:1 or 1:2) and/or use lower protein concentration.
Best regards, Vicky G. Tsirkone, MSc Laboratory for Biocrystallography Department of Pharmaceutical and Pharmacological Sciences KU Leuven O&N II Herestraat 49 - box 822 3000 Leuven | Belgium Tel.: +32 16 3 23419 e-mail: vicky.tsirk...@pharm.kuleuven.be<mailto:vicky.tsirk...@pharm.kuleuven.be> ________________________________ From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] on behalf of Prerana G. [tracy...@gmail.com] Sent: Friday, February 21, 2014 12:14 PM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] Hi, Sorry for asking an off-topic question, I have recently purified a protein having a molecular weight of 40kDa and concentration of the protein was 8mg/ml. When I tried to set the protein for crystallisation using micobatch method, the protein started precipitating in most of the buffer conditions of Crystal screen and Peg ion. The precipitation took place very quickly (within 5-10 mins). How should I overcome this problem? Regards Prerana
