Also see Acta Cryst. (2013). D69, 1433-1446 [ doi:10.1107/S0907444913015126<http://dx.doi.org/10.1107/S0907444913015126> ] Best wishes Elspeth
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Allister Crow Sent: 27 December 2013 21:31 To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] structural homologs as cross seeds I know of at least one published example where seeding has been used to crystallise a number of homologues: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2243101/ (Sanchez-Weatherby 2006, Acta Cryst F 62:518) There are probably others out there... Certainly worth giving it a go. Best wishes, - Allister On 27 December 2013 19:29, Mahesh Lingaraju <mxl1...@psu.edu<mailto:mxl1...@psu.edu>> wrote: Dear all, I was wondering if it sounds logical to use the crystals from a possible structural homolog as seeds to induce nucleation ? (in terms of overall sequence, the proteins are considerably different but based on sequence alignment and structures from other related proteins, it is highly likely the protein would have the same structure.) Please comment if any of you had experience with this. Thank you Happy holidays :) Mahesh
