I know of at least one published example where seeding has been used to crystallise a number of homologues:
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2243101/ (Sanchez-Weatherby 2006, Acta Cryst F 62:518) There are probably others out there... Certainly worth giving it a go. Best wishes, - Allister On 27 December 2013 19:29, Mahesh Lingaraju <mxl1...@psu.edu> wrote: > Dear all, > > I was wondering if it sounds logical to use the crystals from a possible > structural homolog as seeds to induce nucleation ? (in terms of overall > sequence, the proteins are considerably different but based on sequence > alignment and structures from other related proteins, it is highly likely > the protein would have the same structure.) > > Please comment if any of you had experience with this. > > Thank you > > Happy holidays :) > > Mahesh >
