Well said Petri, also how much PEG3350 do you have in your conditions ? More than 25% ? I'm going after cryo-conditions at this point, you might want to replace your PEG3350 with smaller PEGs or a mixture of PEG400 and PEG3350. Almost sounds as if no optimization of the original conditions was performed yet.
Plenty to do for you, also since you have some crystal use them for seeding into your new screens. Jürgen On Aug 19, 2013, at 5:46 PM, Mahesh Lingaraju wrote: Hi Petri They are non-diffracting at the home source and they are cryo cooled. Like david suggested I guess ill try introducing a buffer as my condition does not have a buffer. it is ammonium acetate and PEG 3350. Thanks for the encouragement ! Mahesh On Mon, Aug 19, 2013 at 5:37 PM, Petri Kursula <petri.kurs...@gmail.com<mailto:petri.kurs...@gmail.com>> wrote: Hi, non-diffracting on the home source or state-of-the-art synchrotron? Cryocooled or room-temperature? What happens if you change the buffer but keep your pH? etc etc... For an important project, one should never ever give up. Petri --- Petri Kursula, PhD project leader, adjunct professor Department of Biochemistry & Biocenter Oulu, University of Oulu, Finland Department of Chemistry, University of Hamburg/DESY, Germany www.biochem.oulu.fi/kursula<http://www.biochem.oulu.fi/kursula> www.desy.de/~petri/research<http://www.desy.de/~petri/research> petri.kurs...@oulu.fi<mailto:petri.kurs...@oulu.fi> --- On Aug 19, 2013, at 11:49 PM, Mahesh Lingaraju <mxl1...@psu.edu<mailto:mxl1...@psu.edu>> wrote: Hello people I recently obtained hexagonal rod like crystals (150x50x20 um) which turned out to be non diffracting. What is the usual convention for cases like this ? do people usually give up on the condition or still try to optimize it ? The crystals are also not very reproducible. I believe it is because of ammonium acetate in the condition causing fluctuations in the pH because of its volatility. Is there any way to work around such a problem ? Thanks Mahesh ...................... Jürgen Bosch Johns Hopkins University Bloomberg School of Public Health Department of Biochemistry & Molecular Biology Johns Hopkins Malaria Research Institute 615 North Wolfe Street, W8708 Baltimore, MD 21205 Office: +1-410-614-4742 Lab: +1-410-614-4894 Fax: +1-410-955-2926 http://lupo.jhsph.edu
