Hi Petri They are non-diffracting at the home source and they are cryo cooled. Like david suggested I guess ill try introducing a buffer as my condition does not have a buffer. it is ammonium acetate and PEG 3350.
Thanks for the encouragement ! Mahesh On Mon, Aug 19, 2013 at 5:37 PM, Petri Kursula <petri.kurs...@gmail.com>wrote: > Hi, > > non-diffracting on the home source or state-of-the-art synchrotron? > Cryocooled or room-temperature? What happens if you change the buffer but > keep your pH? etc etc... > > For an important project, one should never ever give up. > > Petri > > > --- > Petri Kursula, PhD > project leader, adjunct professor > Department of Biochemistry & Biocenter Oulu, University of Oulu, Finland > Department of Chemistry, University of Hamburg/DESY, Germany > www.biochem.oulu.fi/kursula > www.desy.de/~petri/research > petri.kurs...@oulu.fi > --- > > > > > > On Aug 19, 2013, at 11:49 PM, Mahesh Lingaraju <mxl1...@psu.edu> wrote: > > Hello people > > I recently obtained hexagonal rod like crystals (150x50x20 um) which > turned out to be non diffracting. What is the usual convention for cases > like this ? do people usually give up on the condition or still try to > optimize it ? > > The crystals are also not very reproducible. I believe it is because of > ammonium acetate in the condition causing fluctuations in the pH because of > its volatility. Is there any way to work around such a problem ? > > Thanks > > Mahesh > > > >
