Thanks for all suggestions. I am going to P32-label the RNA and see if it runs as a single species on the gel and if not, I'll do the HPLC. Alex
On Mon, May 20, 2013 at 11:16 AM, Eugene Valkov <eugene.val...@gmail.com>wrote: > Hi Alex, > > If you do not have access to HPLC equipment, another alternative is > gel-purification using a PAGE setup under denaturing (urea) conditions. > This has the advantage of being fairly simple and effective for a range of > transcripts, but you will need a fairly large gel tank system to get good > resolution. Ke and Doudna 2004 review in Methods is an excellent starting > point for learning about practical issues involved in the crystallisation > of protein/RNA complexes and purification of RNA for this purpose. > > Hope this helps, > Eugene > > > > On 20 May 2013 15:20, A K <alek6...@gmail.com> wrote: > >> Dear all, >> I have a crystallization-related question. I am going to co-crystallize >> protein with RNA. I ordered a short (10 mer and 14 mer) RNA from Dharmacon >> but did not choose the purification option while ordering them due to the >> budget issues. How critical is to HPLC purify them before setting drops? >> Aren't RNA synthesis protocols reliable enough nowadays for such short RNAs? >> Thanks, >> Alex >> > > > > -- > Dr Eugene Valkov > > Division of Structural Studies > MRC Laboratory of Molecular Biology > Francis Crick Avenue > Cambridge Biomedical Campus > Cambridge CB2 0QH, U.K. > > Email: eval...@mrc-lmb.cam.ac.uk > Tel: +44 (0) 1223 407840 >
