Dear Alex, In my experience it is essential to HPLC purify short-RNAs for crystallisations as the impurities will hinder your crystallisation attempts.
Best wishes, Salima Dr. Salima Nurmohamed Division of Structural Biology, Wellcome Trust Centre for Human Genetics, University of Oxford, Roosevelt Drive Oxford OX3 7BN, UK -- Kay Kendall Junior Research Fellow On 20 May 2013, at 15:20, A K <alek6...@gmail.com> wrote: > Dear all, > I have a crystallization-related question. I am going to co-crystallize > protein with RNA. I ordered a short (10 mer and 14 mer) RNA from Dharmacon > but did not choose the purification option while ordering them due to the > budget issues. How critical is to HPLC purify them before setting drops? > Aren't RNA synthesis protocols reliable enough nowadays for such short RNAs? > Thanks, > Alex
