The seminal paper on actually how to collect data from detectors like this
and others with negligible read-out time is this one, which I strongly
recommend:
Optimal Fine phi-slicing for Single-Photon-Counting Pixel Detectors Marcus
Mueller, Meitian Wang, and Clemens Schulze-Briese, Acta Cryst.(2012) D68,
42-56
And you can pick up a copy of the paper from the RapiData web site:
http://www.px.nsls.bnl.gov/courses/papers/Mueller_ACD68_2012.pdf
The classic paper on data-collection strategies is this:
Data-Collection Stragegies, Dauter, Z. Acta Cryst. (1999). D55, 1703-1717.
Also available from the RapiData site:
http://www.px.nsls.bnl.gov/courses/papers/dauter_strategy.pdf
Then there are multiple papers on damage and its impact on your data. I
suggest this one:
Radiation damage in macromolecular crystallography: what is it and why
should we care?, E.Garman, Acta Cryst. D66, 339-351(2010).
which you can find here:
http://www.px.nsls.bnl.gov/courses/papers/actad-garman-2010.pdf
With this under your belt you'll be able to decide how to collect your
phasing data. The bottom line is probably that you should go for SAD
data. Employ multiple crystals and average them together in a judicious
way, keeping only the sweeps from barely damaged x-tals.
Good luck,
Bob Sweet
=========================================================================
Robert M. Sweet E-Dress: sw...@bnl.gov
Group Leader, PXRR: Macromolecular ^ (that's L
Crystallography Research Resource at NSLS not 1)
http://px.nsls.bnl.gov/
Photon Sciences and Biosciences Dept
Office and mail, Bldg 745, a.k.a. LOB-5
Brookhaven Nat'l Lab. Phones:
Upton, NY 11973 631 344 3401 (Office)
U.S.A. 631 344 2741 (Facsimile)
=========================================================================
On Mon, 6 May 2013, Theresa Hsu wrote:
Dear crystallographers
Is there a good source/review/software to obtain tips for good data
collection strategy using PILATUS detectors at synchrotron? Do we need
to collect sweeps of high and low resolution data separately? For
anomalous phasing (MAD), does the order of wavelengths used affect
structure solution or limit radiation damage?
Thank you.
Theresa
