hello everyone, i have a protein which co-purifies with a ligand (small molecule ~ 810 Dalton). i didn't know what kind of ligand is it so i am planing to identify it. meanwhile my protein crystallized as plates and while diffracting i found that the crystal is showing a good diffraction pattern upto 2.6 Angstroms in home source Cu K-alpha. however when the crystal rotates between 90 degrees to 135 degrees, the spots become streaky. should i try different cryo-MPD/ PEG400 etc? to circuvent the problem i did some additive screen but was not of much help. any valuable suggestion willbe handfull.
thanx in advance rakesh
