Qiagen used to sell hanging-drop trays with screw-cap tops, and the tops had six rings on them (3 large, 3 small). The rings had a lip on them that kept the drop from spreading beyond the edge. I found these quite useful when setting up drops that had detergent in them.
I unfortunately don't know what they are called, as I have several at my bench but not the packaging they arrived in. On Wed, Nov 7, 2012 at 9:04 AM, Eva Bligt-Lindén <eva.bl...@abo.fi> wrote: > Thank you for your replies. To my knowledge the protein is not a > surface-tension-reducing-**protein and there is no detergent in the > sample. I have tried different plates and still the same result. I will try > a different crystallization technique such as batch under oil or > counter-diffusion if I do not solve this problem. But now I am curious to > know what is causing the disturbed surface tension and if someone else has > experienced and dealt with something similar. > > Kind regards, > Eva > > > Quoting anna anna <marmottalb...@gmail.com>: > > Why don't you try batch under oil? >> >> 2012/11/7 Eva Bligt-Lindén <eva.bl...@abo.fi> >> >> Dear ccp4 users, >>> >>> I have a problem in the crystallization of my target protein. Whenever I >>> set up a vapour diffusion experiment, either hanging or sitting drops, >>> the >>> drops spread out. The surface tension is completely lost in 80-90% of the >>> droplets. Have any one experienced something similar? What could be the >>> reason for this strange behaviour? I have tried three different >>> commercial >>> screens with 96 condition each and there is no difference between the >>> screens. There is no difference between manual or robotic setups either. >>> The protein buffer is 40 mM Tris, 2 mM MgCl2 buffer, pH 7.4. The buffer >>> controls are all ok. >>> >>> Kind regards, >>> Eva >>> >>> ______________________________****______ >>> >>> >>> Eva Bligt-Lindén (M.Sc.) >>> PhD student >>> Structural Bioinformatics Laboratory >>> >>> Department of Biosciences, >>> Åbo Akademi University >>> BioCity, Tykistökatu 6A >>> FI-20520 Turku >>> Finland >>> >>> >> > > > ______________________________**______ > > Eva Bligt-Lindén (M.Sc.) > PhD student > Structural Bioinformatics Laboratory > > Department of Biosciences, > Åbo Akademi University > BioCity, Tykistökatu 6A > FI-20520 Turku > Finland >
