is your protein a STRP (surface-tension-reducing protein)? Seriously, did you try different plates? Old-fashioned siliconised cover slips on Linbro plates?
Does your sample contain detergent? Mark J van Raaij Laboratorio M-4 Dpto de Estructura de Macromoleculas Centro Nacional de Biotecnologia - CSIC c/Darwin 3 E-28049 Madrid, Spain tel. (+34) 91 585 4616 http://www.cnb.csic.es/~mjvanraaij On 7 Nov 2012, at 13:07, Eva Bligt-Lindén wrote: > Dear ccp4 users, > > I have a problem in the crystallization of my target protein. Whenever I set > up a vapour diffusion experiment, either hanging or sitting drops, the drops > spread out. The surface tension is completely lost in 80-90% of the droplets. > Have any one experienced something similar? What could be the reason for this > strange behaviour? I have tried three different commercial screens with 96 > condition each and there is no difference between the screens. There is no > difference between manual or robotic setups either. The protein buffer is 40 > mM Tris, 2 mM MgCl2 buffer, pH 7.4. The buffer controls are all ok. > > Kind regards, > Eva > > ____________________________________ > > Eva Bligt-Lindén (M.Sc.) > PhD student > Structural Bioinformatics Laboratory > > Department of Biosciences, > Åbo Akademi University > BioCity, Tykistökatu 6A > FI-20520 Turku > Finland
