Hi Tanya - did you read the next message in the thread you posted? It
answers the question, even if somewhat succinctly:
http://www.proteincrystallography.org/ccp4bb/message23692.html
The April 2011 issue of Journal of Synchrotron Radiation has the
proceedings from the 2010 Radiation Damage workshop; those papers and
their references are an excellent starting point if you want to know
more of the gory details.
Cheers
phx
On 02/08/2012 23:50, David Schuller wrote:
I do not have the reference you are seeking, but I have seen
cacodylate-containing xtals diffract to better than 1.2 and hold up
very well. Also, arsenic has an anomalous signal which may be
exploited for phasing, peak ~ 1.04 A.
On 07/29/12 18:53, Tatyana Sysoeva wrote:
Hi!
I heard a couple of times that use of cacodylate buffers in
crystallization is bad, and not only because of the compound toxicity.
As I understood, presence of the cacodylate in a protein crystal will
cause a particular crystal degradation pattern upon X-ray exposure -
"darkening of the crystals, gas formation"
I tried to find some references on that and failed in doing so.
I found some earlier discussions like this one:
http://www.proteincrystallography.org/ccp4bb/message23691.html
but don't have anything to reference in literature. I would
appreciate if someone can point me to a right direction.
I am sorry if this question is out of the groups topic range.
Thank you in advance!
Sincerely,
Tanya
--
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All Things Serve the Beam
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David J. Schuller
modern man in a post-modern world
MacCHESS, Cornell University
schul...@cornell.edu
