I do not have the reference you are seeking, but I have seen
cacodylate-containing xtals diffract to better than 1.2 and hold up very
well. Also, arsenic has an anomalous signal which may be exploited for
phasing, peak ~ 1.04 A.
On 07/29/12 18:53, Tatyana Sysoeva wrote:
Hi!
I heard a couple of times that use of cacodylate buffers in
crystallization is bad, and not only because of the compound toxicity.
As I understood, presence of the cacodylate in a protein crystal will
cause a particular crystal degradation pattern upon X-ray exposure -
"darkening of the crystals, gas formation"
I tried to find some references on that and failed in doing so.
I found some earlier discussions like this one:
http://www.proteincrystallography.org/ccp4bb/message23691.html
but don't have anything to reference in literature. I would appreciate
if someone can point me to a right direction.
I am sorry if this question is out of the groups topic range.
Thank you in advance!
Sincerely,
Tanya
--
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All Things Serve the Beam
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David J. Schuller
modern man in a post-modern world
MacCHESS, Cornell University
schul...@cornell.edu
