Hi! I heard a couple of times that use of cacodylate buffers in crystallization is bad, and not only because of the compound toxicity.
As I understood, presence of the cacodylate in a protein crystal will cause a particular crystal degradation pattern upon X-ray exposure - "darkening of the crystals, gas formation" I tried to find some references on that and failed in doing so. I found some earlier discussions like this one: http://www.proteincrystallography.org/ccp4bb/message23691.html but don't have anything to reference in literature. I would appreciate if someone can point me to a right direction. I am sorry if this question is out of the groups topic range. Thank you in advance! Sincerely, Tanya
