If you have a CD available (not the one with music on it) you don't need a dye just sufficient protein and you can thermal denature your protein assuming it contains some secondary structure elements.
Jürgen On Jul 19, 2012, at 4:26 AM, anita p wrote: Hi All, I want to use a thermofluor for the thermal shift assay. My proteins are cytoplasmic truncations of membrane protein. I have read about ANS, sypro-orange and CPM. Which is the once that is popularly used by the crystallographers for condition optimization for crystallization ?? I have read that it sypro orange is not good for hydrophobic proteins and CPM can't be used with DTT or bME in the buffer. I am a bit confused . Please help thanks in advance Anita ...................... Jürgen Bosch Johns Hopkins University Bloomberg School of Public Health Department of Biochemistry & Molecular Biology Johns Hopkins Malaria Research Institute 615 North Wolfe Street, W8708 Baltimore, MD 21205 Office: +1-410-614-4742 Lab: +1-410-614-4894 Fax: +1-410-955-2926 http://lupo.jhsph.edu
