Hi All, I want to use a thermofluor for the thermal shift assay. My proteins are cytoplasmic truncations of membrane protein. I have read about ANS, sypro-orange and CPM. Which is the once that is popularly used by the crystallographers for condition optimization for crystallization ??
I have read that it sypro orange is not good for hydrophobic proteins and CPM can't be used with DTT or bME in the buffer. I am a bit confused . Please help thanks in advance Anita
