Dear Anita,
here is a link to a talk with recommended literature:
http://thermofluor.org/resources/Maksel_Protein-Stability.pdf.
Probably, you will find there the answers you need.
I recommend to try several dyes if available, due to the different
behaviour of proteins and no general rule for doing thermal shift assays.
good luck
Hüsnü Topal, Dr. rer. nat.
Protein Crystallography & Bioinformatics
Workgroup of Prof. Dr. W. Welte
Universitätsstr. 10
78457 Konstanz
Am 19.07.2012 10:26, schrieb anita p:
Hi All,
I want to use a thermofluor for the thermal shift assay. My proteins
are cytoplasmic truncations of membrane protein. I have read about
ANS, sypro-orange and CPM. Which is the once that is popularly used by
the crystallographers for condition optimization for crystallization ??
I have read that it sypro orange is not good for hydrophobic proteins
and CPM can't be used with DTT or bME in the buffer.
I am a bit confused .
Please help
thanks in advance
Anita
