I can only confirm what Alex said. And the structure was neither a
globin or zyme or psin!
Victor
Quoting aaleshin <aales...@burnham.org>:
I and Victor Lamzin solved our first protein structure (3A
resolution) in 80-s using pure MIR and a home made (Russian)
diffractometer...
Alex
On Jun 6, 2012, at 1:42 PM, Boaz Shaanan wrote:
So if get the gist of the thread right, am I correct in assuming
that the last protein structures to be solved strictly by MIR are
haemoglobin/myoglobin, lysozyme and chymotrypsin and perhaps one or
two more in the late sixties? In which case the answer to the
original question about MIR being obsolete, is "yes it is since a
long time"?
Boaz
Boaz Shaanan, Ph.D.
Dept. of Life Sciences
Ben-Gurion University of the Negev
Beer-Sheva 84105
Israel
E-mail: bshaa...@bgu.ac.il
Phone: 972-8-647-2220 Skype: boaz.shaanan
Fax: 972-8-647-2992 or 972-8-646-1710
________________________________________
From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] on behalf of Phil
Evans [p...@mrc-lmb.cam.ac.uk]
Sent: Wednesday, June 06, 2012 6:04 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Fun Question - Is multiple isomorphous
replacement an obsolete technique?
No they were not useless! I used them
(probably better now with cryo data though)
Phil
On 6 Jun 2012, at 16:02, Dyda wrote:
I suspect that pure MIR (without anomalous) was always a fiction.
I doubt that anyone has ever used it. Heavy atoms always give
an anomalous signal
Phil
I suspect that there was a time when the anomalous signal in data
sets was fictional.
Before the invent of flash freezing, systematic errors due to
decay and the need
of scaling together many derivative data sets collected on
multiple crystals could render
weak anomalous signal useless. Therefore MIR was needed. Also,
current hardware/software
produces much better reduced data, so weak signals can become useful.
Fred
?[32m*******************************************************************************
Fred Dyda, Ph.D. Phone:301-402-4496
Laboratory of Molecular Biology Fax: 301-496-0201
DHHS/NIH/NIDDK e-mail:fred.d...@nih.gov
Bldg. 5. Room 303
Bethesda, MD 20892-0560 URGENT message e-mail: 2022476...@mms.att.net
Google maps coords: 39.000597, -77.102102
http://www2.niddk.nih.gov/NIDDKLabs/IntramuralFaculty/DydaFred
*******************************************************************************?[m
