For those who want to stay with the CCP4 GUI, yu can rn SHELXC/D/E from it
and get the advantage of keeping all info in the same place.. The Zn edge
is _ 1.28 but you can use the Zn signal for any wavelength less than that..
Eleanor
On Mar 6 2012, Bosch, Juergen wrote:
Since you've collected the data already use your favourite data
processing program and treat the Friedel pairs separately. I'd suggest to
try HKL2map in conjunction with SHELX C/D/E (sorry for the non CCP4
advertisement here) for solving the heavy atom sites. You can in parallel
also try SnB or BnP to find a substructure solution. Depending how bad
you resulting density looks like you might want to improve your phases
via Sharp.
If you want to stay in the CCP4 protected sandbox, then give Crank a try.
Jürgen
On Mar 6, 2012, at 3:24 PM, Francis E Reyes wrote:
http://skuld.bmsc.washington.edu/scatter/AS_form.html
Maybe useful to you.
However, I would advise to do a fluorescence scan over the range given in
the graph and then use chooch to provide the precise energies for your
peak and inflection.
If you have a large crystal don't expose all of it when you do the
fluorescence scan but rather reserve a 'fresh' piece to do your actual
data collection.
F
On Mar 6, 2012, at 1:09 PM, Deepthi wrote:
Hi
I am trying to solve the structure of an engineered protein.The protein
is crystallized with Zn bound to it .We collected a 1.5A0 data. Molecular
Replacement didn't yield a good match for the protein. I want to try MAD
taking advantage of the Zn atoms in protein. I am not sure what
wavelength should i use to collect the diffraction data for Zn. any
suggestions?
Thank You
Deepthi
--
Deepthi
......................
Jürgen Bosch
Johns Hopkins University
Bloomberg School of Public Health
Department of Biochemistry & Molecular Biology
Johns Hopkins Malaria Research Institute
615 North Wolfe Street, W8708
Baltimore, MD 21205
Office: +1-410-614-4742
Lab: +1-410-614-4894
Fax: +1-410-955-2926
http://web.mac.com/bosch_lab/
--
Professor Eleanor Dodson
YSNL, Dept of Chemistry
University of York
Heslington YO10 5YW
tel: 00 44 1904 328259
Fax: 00 44 1904 328266
