A reference for a real MAD phasing with Zinc (worked very well):
Ennifar et al. MAD phasing replacing magnesium with zinc. Acta Cryst.
(2001). D57, 330
Philippe Dumas
"Bosch, Juergen" <jubo...@jhsph.edu> a écrit :
Since you've collected the data already use your favourite data
processing program and treat the Friedel pairs separately. I'd
suggest to try HKL2map in conjunction with SHELX C/D/E (sorry for
the non CCP4 advertisement here) for solving the heavy atom sites.
You can in parallel also try SnB or BnP to find a substructure
solution. Depending how bad you resulting density looks like you
might want to improve your phases via Sharp.
If you want to stay in the CCP4 protected sandbox, then give Crank a try.
Jürgen
On Mar 6, 2012, at 3:24 PM, Francis E Reyes wrote:
http://skuld.bmsc.washington.edu/scatter/AS_form.html
Maybe useful to you.
However, I would advise to do a fluorescence scan over the range
given in the graph and then use chooch to provide the precise
energies for your peak and inflection.
If you have a large crystal don't expose all of it when you do the
fluorescence scan but rather reserve a 'fresh' piece to do your
actual data collection.
F
On Mar 6, 2012, at 1:09 PM, Deepthi wrote:
Hi
I am trying to solve the structure of an engineered protein.The
protein is crystallized with Zn bound to it .We collected a 1.5A0
data. Molecular Replacement didn't yield a good match for the
protein. I want to try MAD taking advantage of the Zn atoms in
protein. I am not sure what wavelength should i use to collect the
diffraction data for Zn. any suggestions?
Thank You
Deepthi
--
Deepthi
......................
Jürgen Bosch
Johns Hopkins University
Bloomberg School of Public Health
Department of Biochemistry & Molecular Biology
Johns Hopkins Malaria Research Institute
615 North Wolfe Street, W8708
Baltimore, MD 21205
Office: +1-410-614-4742
Lab: +1-410-614-4894
Fax: +1-410-955-2926
http://web.mac.com/bosch_lab/
