Self-referentially:
I once used the structural Zn of p53 to do a Zn MAD structure of a
p53:53BP1 complex at 2.5 Angstrom with one zinc per 450 residues.
Apparently using 1.283, 1.282 and 1.262 Angstroms (i.e. the Zinc edge).
http://genesdev.cshlp.org/content/16/5/583.long
But of course do your own fluorescence scan. The advantage of
structural metals is full occupancy and relatively lower B-factor.
That map was actually pretty good, and since it came out of MLPHARE I
don't doubt modern programs like SHARP could make it quite a lot better.
Phil Jeffrey
Princeton
On 3/6/12 3:09 PM, Deepthi wrote:
Hi
I am trying to solve the structure of an engineered protein.The protein
is crystallized with Zn bound to it .We collected a 1.5A0 data.
Molecular Replacement didn't yield a good match for the protein. I want
to try MAD taking advantage of the Zn atoms in protein. I am not sure
what wavelength should i use to collect the diffraction data for Zn. any
suggestions?
Thank You
Deepthi
--
Deepthi
