If you want test a lot of different conditions a Thermofluorecence assay might work for your protein and you may find a condition which stabilise your protein. However there is no warranty that it crystallise better afterwards.
Christian Am Montag 27 Februar 2012 17:01:33 schrieb Sangeetha Vedula: > Dear bb users, > > I am trying to crystallize a ~320 kDa protein that crashes out if > concentrated past about 3 mg/mL. > > I would like to try to exchange it into various buffer-salt-additive > combinations to see which buffer works. For a starting point, I'd like to > use desalting colums. > > Does anyone have suggestions for good buffer exchange and sample recovery? > I woud like to load about 250 uL onto each column. > > Thanks a lot! > > Best regards, > > Sangeetha. >
