Hi Sangeetha:
If you just want to check which buffer is good for your protein, maybe you can
try to set up a crystallization screen, keeping your protein concentration just
3 mg/ml. You can observe (after several days) which conditions give you a clear
drop, and maybe you can find a clue which buffer is better for your protein. If
you want to speed up the whole processes, you can also add glycerol into the
drops to grasp the water molecules.
Yu Xiaodi
Date: Mon, 27 Feb 2012 11:01:33 -0500
From: sangeetha...@gmail.com
Subject: [ccp4bb] Desalting columns
To: CCP4BB@JISCMAIL.AC.UK
Dear bb users,
I am trying to crystallize a ~320 kDa protein that crashes out if concentrated
past about 3 mg/mL.
I would like to try to exchange it into various buffer-salt-additive
combinations to see which buffer works. For a starting point, I'd like to use
desalting colums.
Does anyone have suggestions for good buffer exchange and sample recovery? I
woud like to load about 250 uL onto each column.
Thanks a lot!
Best regards,
Sangeetha.
