Dear all,
I am trying to crystallize a protein kinase without any success. I suspect about its characteristic catalytic loop. I have already prepared different constructs, different expression vectors, and different mutant proteins (pseudo-phosphorylated, active, inactive?). I have also tested some ligands as ANPpnp (non hidrolizable nucleotide), ADP (product) and manganese (cofactor). I am thinking in trying to cocrystallize it with a general kinase substrate (a peptide, a small molecule...). Does any one have any experience or suggestion? Thanks in advance. Sincerely, Antonio
