It is a fairly common issue with kinases. Among other options you may want to try a generic kinase inhibitor (there are several good ones just look at pdb structures for ieas) and if this does not help then you could attempt to clamp the motion down via an inter-lobe engineered disulphide bond...
Artem On Jan 30, 2012 5:17 AM, "CHAVES SANJUAN, ANTONIO" <xanto...@iqfr.csic.es> wrote: > Dear all,** > > I am trying to crystallize a protein kinase without any success.** > > I suspect about its characteristic catalytic loop. I have already prepared > different constructs, different expression vectors, and different mutant > proteins (pseudo-phosphorylated, active, inactive?). I have also tested > some ligands as ANPpnp (non hidrolizable nucleotide), ADP (product) and > manganese (cofactor).** > > I am thinking in trying to cocrystallize it with a general kinase > substrate (a peptide, a small molecule...). Does any one have any > experience or suggestion?** > > Thanks in advance.** > > Sincerely,** > Antonio
